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Chin. Opt. Lett.
 Home  List of Issues    Issue 01 , Vol. 04 , 2006    Determining particle size distribution and refractive index in a two-layer tissue phantom by linearly polarized light


Determining particle size distribution and refractive index in a two-layer tissue phantom by linearly polarized light
Yong Deng, Qiang Lu, Qingming Luo
Key Laboratory of Biomedical Photonics of Ministry of Education, [Huazhong University of Science and Technology], Wuhan 430074

Chin. Opt. Lett., 2006, 04(01): pp.45-48-4

DOI:
Topic:Medical optics and biotechnology
Keywords(OCIS Code): 170.3660  260.5430  290.1350  290.4020  

Abstract
We report a new method for measuring particle size distribution (PSD) and refractive index of the top layer in a two-layer tissue phantom simulated epithelium tissue by varying the azimuth angle of incident linearly polarized light. The polarization gating technique is used to decouple the single and multiple scattering components in the returned signal. The theoretical model based on Mie theory is presented and a nonlinear inversion method -- floating genetic algorithm -- is applied to inverting the azimuth dependence of component of polarization light backscattered. The experiment results demonstrate that the size distribution and refractive index of the scatters of the top layer can be determined by measuring and analyzing the differential signal of the parallel and perpendicular components from a two-layer tissue phantom. The method implies to detect precancerous changes in human epithelial tissue.

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Received:2005/7/11
Accepted:
Posted online:

Get Citation: Yong Deng, Qiang Lu, Qingming Luo, "Determining particle size distribution and refractive index in a two-layer tissue phantom by linearly polarized light," Chin. Opt. Lett. 04(01), 45-48-4(2006)

Note: This work was carried out at the Key Laboratory of Biomedical Photonics of Ministry of Education, Huazhong University of Science and Technology. This research was supported by the National Natural Science Foundation of China (No. 30470460 and 60278017). The author would like to thank M.S. Rui Hu who helped with the experiment and Dr. JuQiang Lin for generously providing the HeLa cells. Q. Luo is the author to whom the correspondence should be addressed, his e-mail address is qluo@mail.hust.edu.cn.



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