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Chin. Opt. Lett.
 Home  List of Issues    Issue 11 , Vol. 13 , 2015    10.3788/COL201513.111701

Imaging of nerve injury with HSP27 antibody-functionalized photoacoustic nanoprobes
Hongjiang Chen1, Zhongjiang Chen2, Jankun Xu1, and Jun Hu1;2
1 Department of Orthopaedics, the First Affiliated Hospital, [Shantou University Medical College], Shantou 51 5041 , China
2 MOE Key Laboratory of Laser Life Science &
Institute of Laser Life Science, College of Biophotonics, [South China Normal University], Guangzhou 510631, China

Chin. Opt. Lett., 2015, 13(11): pp.111701

Topic:Medical optics and biotechnology
Keywords(OCIS Code): 170.0170  170.3880  170.5120  

Early detection and timely treatment of nerve injury is crucial for the repair of nerve function. One week following a crush injury, heat shock protein 27 (HSP27) is over-expressed along the entire length of the sciatic nerve. Herein, we present an approach to detect injured nerves by photoacoustic microscopy after labeling the injured nerve with HSP27 antibody-conjugated gold nanoparticles. The studies reveal that nanoprobe administration enabled the detection of injured nerves by photoacoustic microscopy, especially during the early stages within 3–7 days post injury. In conclusion, photoacoustic microscopy combined with antibody-conjugated nanoparticles holds potential for the early detection of nerve injury.

Copyright: © 2003-2012 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Posted online:2015/10/16

Get Citation: Hongjiang Chen, Zhongjiang Chen, Jankun Xu, and Jun Hu, "Imaging of nerve injury with HSP27 antibody-functionalized photoacoustic nanoprobes," Chin. Opt. Lett. 13(11), 111701(2015)

Note: This work was supported by the National Natural Science Foundation of China (No.?81271619), the Natural Science Foundation of Guangdong Province (No.?10151503102000016), the National High Technology Research and Development Program of China (No.?2015AA020901), the Science and Technology Planning Project of Guangdong Province (No.?2013B090500122), and the Guangdong Natural Science Foundation (No.?S2013020012646).


1. R. N. Kalaria, Nutr. Rev.68, S74 (2010).

2. W. W. Campbell, Clin. Neurophysiol.119, 1951 (2008).

3. A. V. Korompilias, A. H. Payatakes, A. E. Beris, M. D. Vekris, G. D. Afendras, and P. N. Soucacos, J. Microsurg.26, 288 (2006).

4. J. Zhu, F. Liu, D. Li, J. Shao, and B. Hu, Eur. J. Radiol.21, 1097 (2011).

5. D. D. Linda, S. Harish, B. G. Stewart, K. Finlay, N. Parasu, and R. P. Rebello, RG30, 1373 (2010).

6. W. S. Cho, M. Cho, J. Jeong, M. Choi, H. Y. Cho, B. S. Han, S. H. Kim, H. O. Kim, Y. T. Lim, and B. H. Chung, Toxicol. Appl. Pharmacol.236, 16 (2009).

7. H. Qin, T. Zhou, S. Yang, Q. Chen, and D. Xing, Nanomedicine (Lond)8, 1611 (2013).

8. M. Prato, K. Kostarelos, and A. Bianco, Acc. Chem. Res.41, 60 (2008).

9. S. K. Sahoo, S. Parveen, and J. J. Panda, Nanomedicine3, 20 (2007).

10. J. Zhang, S. Yang, X. Ji, Q. Zhou, and D. Xing, J. Am. Coll. Cardiol.64, 385 (2014).

11. B. Li, H. Qin, S. Yang, and D. Xing, Opt. Express22, 20130 (2014).

12. Y. Wang, J. Yuan, S. Du, X. Liu, G. Xu, and X. Wang, Chin. Opt. Lett.13, 061001 (2015).

13. Z. Wu, M. Sun, Q. Wang, T. Liu, N. Feng, J. Liu, and Y. Shen, Chin. Opt. Lett.12, 121701 (2014).

14. Z. Chen, S. Yang, and D. Xing, Opt. Lett.37, 3414 (2012).

15. S. Yang, F. Ye, and D. Xing, Opt. Express20, 10370 (2012).

16. S. Yang, D. Xing, Q. Zhou, L. Xiang, and Y. Lao, Med. Phys.34, 3294 (2007).

17. G. Liu, and Z. Chen, Chin. Opt. Lett.11, 11702 (2013).

18. P. Mehlen, C. Kretz-Remy, X. Preville, and A. P. Arrigo, EMBO J.15, 2695 (1996).

19. J. N. Lavoie, G. Gingras-Breton, R. M. Tanguay, and J. Landry, J. Biol. Chem.268, 3420 (1993).

20. M. Costigan, R. J. Mannion, G. Kendall, S. E. Lewis, J. A. Campagna, R. E. Coggeshall, J. Meridith-Middleton, S. Tate, and C. J. Woolf, J. Neurosci.18, 5891 (1998).

21. K. Hirata, J. He, Y. Hirakawa, W. Liu, S. Wang, and M. Kawabuchi, Glia42, 1 (2003).

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